MicroRNA hsa-miR-125a-3p activates p53 and induces apoptosis in lung cancer cells

Cancer Invest. 2013 Oct;31(8):538-44. doi: 10.3109/07357907.2013.820314. Epub 2013 Sep 18.


The mature microRNA hsa-miR-125a-3p is derived from the 3' end of pre-miR-125a. Here, we reported that hsa-miR-125a-3p suppressed proliferation and induced apoptosis in A549 cells. In addition, wild-type p53 mRNA and protein expression was increased by hsa-miR-125a-3p over-expression. Moreover, blocking wild-type p53 attenuated the effect of hsa-miR-125a-3p on apoptosis but could not restore completely. In p53-deficient cell line H1299, hsa-miR-125a-3p still induced apoptosis. Taken together, these data suggest that hsa-miR-125a-3p induces apoptosis not only via the p53 pathway in human lung cancer cells. These results provide new insight into the roles of the miR-125a family in lung cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Apoptosis
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Enzyme Activation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung / metabolism
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / immunology
  • Tumor Suppressor Protein p53 / metabolism*


  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • MIRN125 microRNA, human
  • MicroRNAs
  • TP53 protein, human
  • Tumor Suppressor Protein p53