Anatomical dissection of zebrafish brain development

Methods Mol Biol. 2014;1082:197-214. doi: 10.1007/978-1-62703-655-9_14.

Abstract

Zebrafishbrain.org is an online neuroanatomical atlas of the embryonic zebrafish. The atlas uses high-resolution confocal images and movies of transgenic lines to describe different brain structures. This chapter covers detail of materials and protocols that we employ to generate data for the atlas.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Brain / anatomy & histology*
  • Brain / embryology*
  • Brain / metabolism
  • Culture Techniques
  • Dissection
  • Fluorescent Antibody Technique
  • Fluorescent Dyes / chemistry
  • Microscopy, Confocal
  • Neuroanatomy / methods*
  • Tissue Fixation
  • Zebrafish / anatomy & histology*
  • Zebrafish / embryology*
  • Zebrafish / genetics

Substances

  • Fluorescent Dyes