Evaluation of Isolator System and Large-Volume Centrifugation Method for Culturing Body Fluids

J Clin Microbiol. 1990 Jan;28(1):124-5.

Abstract

The Isolator system was compared with the large-volume centrifugation method for processing and recovering organisms from body fluids other than blood, cerebrospinal fluid, and urine. A total of 155 body fluid samples were processed for the recovery of clinically significant organisms. Of the 55 positive cultures, Isolator detected 94% and the large-volume centrifugation method detected 64%. The time necessary to indicate positivity was not significantly different in the two methods; however, in five cases, the Isolator system yielded clinically significant organisms 24 h sooner than the conventional method. The Isolator system was found to be a more sensitive alternative than the conventional large-volume centrifugation method.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / isolation & purification
  • Body Fluids / microbiology*
  • Centrifugation
  • Evaluation Studies as Topic
  • Humans
  • Microbiological Techniques*