doi: 10.1021/sb400021j.
Epub 2013 May 20.
One-step cloning and chromosomal integration of DNA
Affiliations
- PMID: 24050148
- DOI: 10.1021/sb400021j
Item in Clipboard
One-step cloning and chromosomal integration of DNA
ACS Synth Biol.
.
Abstract
We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
Similar articles
-
A novel approach for Escherichia coli genome editing combining in vivo cloning and targeted long-length chromosomal insertion.J Microbiol Methods. 2016 Nov;130:83-91. doi: 10.1016/j.mimet.2016.08.024. Epub 2016 Aug 25. J Microbiol Methods. 2016. PMID: 27567891
-
Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.Methods Mol Biol. 2017;1472:139-55. doi: 10.1007/978-1-4939-6343-0_11. Methods Mol Biol. 2017. PMID: 27671938
-
Construction of a cloning vector from a naturally occurring plasmid of Salmonella typhimurium.Plasmid. 1995 Sep;34(2):144-7. doi: 10.1006/plas.1995.9993. Plasmid. 1995. PMID: 8559802
-
Binary vectors and super-binary vectors.Methods Mol Biol. 2006;343:15-41. doi: 10.1385/1-59745-130-4:15. Methods Mol Biol. 2006. PMID: 16988331 Review.
-
New methods in Salmonella genetics.Microbiologia. 1994 Dec;10(4):357-70. Microbiologia. 1994. PMID: 7772291 Review.
Cited by
-
A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies.Sci Rep. 2015 Apr 8;5:9684. doi: 10.1038/srep09684. Sci Rep. 2015. PMID: 25851494 Free PMC article.
-
Acoustic biosensors for ultrasound imaging of enzyme activity.Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. Nat Chem Biol. 2020. PMID: 32661379 Free PMC article.
-
Addressable and adaptable intercellular communication via DNA messaging.Nat Commun. 2023 Apr 24;14(1):2358. doi: 10.1038/s41467-023-37788-z. Nat Commun. 2023. PMID: 37095088 Free PMC article.
-
An att site-based recombination reporter system for genome engineering and synthetic DNA assembly.BMC Biotechnol. 2017 Jul 14;17(1):62. doi: 10.1186/s12896-017-0382-1. BMC Biotechnol. 2017. PMID: 28705159 Free PMC article.
-
Programmable DNA looping using engineered bivalent dCas9 complexes.Nat Commun. 2017 Nov 20;8(1):1628. doi: 10.1038/s41467-017-01873-x. Nat Commun. 2017. PMID: 29158476 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
