One-step cloning and chromosomal integration of DNA

ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.


We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.

Publication types

  • Research Support, Non-U.S. Gov't
  • Technical Report

MeSH terms

  • Chromosomes, Bacterial / genetics*
  • Cloning, Molecular / methods*
  • DNA / genetics*
  • DNA Primers
  • Escherichia coli / genetics*
  • Genetic Engineering / methods
  • Genetic Vectors
  • Salmonella typhimurium / genetics*
  • Transformation, Genetic


  • DNA Primers
  • DNA