A propofol binding site on mammalian GABAA receptors identified by photolabeling

Nat Chem Biol. 2013 Nov;9(11):715-20. doi: 10.1038/nchembio.1340. Epub 2013 Sep 22.


Propofol is the most important intravenous general anesthetic in current clinical use. It acts by potentiating GABAA (γ-aminobutyric acid type A) receptors, but where it binds to this receptor is not known and has been a matter of some debate. We synthesized a new propofol analog photolabeling reagent whose biological activity is very similar to that of propofol. We confirmed that this reagent labeled known propofol binding sites in human serum albumin that have been identified using X-ray crystallography. Using a combination of protiated and deuterated versions of the reagent to label mammalian receptors in intact membranes, we identified a new binding site for propofol in GABAA receptors consisting of both β3 homopentamers and α1β3 heteropentamers. The binding site is located within the β subunit at the interface between the transmembrane domains and the extracellular domain and lies close to known determinants of anesthetic sensitivity in the transmembrane segments TM1 and TM2.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Humans
  • Models, Molecular
  • Molecular Structure
  • Photoaffinity Labels / analysis*
  • Photoaffinity Labels / chemistry
  • Propofol / chemistry
  • Propofol / metabolism*
  • Receptors, GABA-A / chemistry*
  • Receptors, GABA-A / metabolism*
  • Serum Albumin / chemistry
  • Structure-Activity Relationship


  • Photoaffinity Labels
  • Receptors, GABA-A
  • Serum Albumin
  • Propofol

Associated data

  • PubChem-Substance/163835817
  • PubChem-Substance/163835818
  • PubChem-Substance/163835819
  • PubChem-Substance/163835820
  • PubChem-Substance/163835821
  • PubChem-Substance/163835822
  • PubChem-Substance/163835823
  • PubChem-Substance/163835824
  • PubChem-Substance/163835825
  • PubChem-Substance/163835826
  • PubChem-Substance/163835827
  • PubChem-Substance/163835828
  • PubChem-Substance/163835829
  • PubChem-Substance/163835830
  • PubChem-Substance/163835831
  • PubChem-Substance/163835832
  • PubChem-Substance/163835833
  • PubChem-Substance/163835834
  • PubChem-Substance/163835835
  • PubChem-Substance/163835836
  • PubChem-Substance/163835837
  • PubChem-Substance/163835838
  • PubChem-Substance/163835839
  • PubChem-Substance/163835840
  • PubChem-Substance/163835841
  • PubChem-Substance/163835842
  • PubChem-Substance/163835843
  • PubChem-Substance/163835844
  • PubChem-Substance/163835845
  • PubChem-Substance/163835846
  • PubChem-Substance/163835847
  • PubChem-Substance/163835848
  • PubChem-Substance/163835849