Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia

Nat Methods. 2013 Nov;10(11):1105-7. doi: 10.1038/nmeth.2647. Epub 2013 Sep 22.


Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Signaling / genetics*
  • Cilia / metabolism*
  • Mice
  • Signal Transduction