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, 10 (11), 1096-8

Smart-seq2 for Sensitive Full-Length Transcriptome Profiling in Single Cells

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Smart-seq2 for Sensitive Full-Length Transcriptome Profiling in Single Cells

Simone Picelli et al. Nat Methods.

Abstract

Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. Here, we introduce Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells. Smart-seq2 transcriptome libraries have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.

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