Smart-seq2 for Sensitive Full-Length Transcriptome Profiling in Single Cells

Nat Methods. 2013 Nov;10(11):1096-8. doi: 10.1038/nmeth.2639. Epub 2013 Sep 22.

Abstract

Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. Here, we introduce Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells. Smart-seq2 transcriptome libraries have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Complementary / genetics
  • Gene Expression Profiling*
  • HEK293 Cells
  • Humans
  • Polymerase Chain Reaction
  • Single-Cell Analysis*
  • Transcriptome*

Substances

  • DNA, Complementary

Associated data

  • GEO/GSE49321