Abstract
We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aorta, Thoracic
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Blotting, Northern
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Cells, Cultured
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Endothelins
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism*
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Gene Expression / drug effects
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Humans
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Indomethacin / pharmacology
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Interleukin-1 / pharmacology*
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Kinetics
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Peptide Biosynthesis*
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Peptides / genetics
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Platelet Activating Factor / antagonists & inhibitors
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Pyridinium Compounds / pharmacology
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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Recombinant Proteins / pharmacology
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Swine
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Transcription, Genetic / drug effects
Substances
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Endothelins
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Interleukin-1
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Peptides
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Platelet Activating Factor
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Pyridinium Compounds
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RNA, Messenger
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Recombinant Proteins
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CV 6209
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Indomethacin