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. 2013 Oct;3(10):825-9.
doi: 10.1016/S2221-1691(13)60163-X. Epub 2013 Sep 4.

First Detection of Leishmania Infantum DNA in Wild Caught Phlebotomus Papatasi in Endemic Focus of Cutaneous Leishmaniasis, South of Iran

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Free PMC article

First Detection of Leishmania Infantum DNA in Wild Caught Phlebotomus Papatasi in Endemic Focus of Cutaneous Leishmaniasis, South of Iran

Rassi Yavar et al. Asian Pac J Trop Biomed. .
Free PMC article

Abstract

Objective: To identify the vectors and reservoirs of cutaneous leishmaniasis in the endemic focus of Farashband, Fars Province, South of Iran.

Methods: Sticky papers and Sherman trap were used for collection of sand flies and rodents, respectively. Polymerase chain reaction (PCR) of kDNA, ITS1-rDNA were used for identification of Leishmania parasite in sand flies as well as rodents.

Results: Totally 2 010 sand flies were collected and the species of Phlebotomus papatasi Scopoli was the common specimen in outdoors and indoors places. PCR technique was employed on 130 females of Phlebotomus papatasi. One of them (0.76%) was positive to parasite Leishmania major (L. major) and one specimen (0.76%) was positive to Leishmania infantum. Microscopic investigation on blood smear of the animal reservoirs for amastigote parasites revealed 16 (44%) infected Tatera indica. Infection of them to L. major was confirmed by PCR against kDNA loci of the parasite.

Conclusions: The results indicated that Phlebotomus papatasi was the dominant species circulating two species of parasites including L. major and Leishmania infantum among human and reservoirs. Furthermore, Tatera indica is the only main host reservoir for maintenance of the parasite source in the area.

Keywords: Iran; Leishmania infantum; Leishmania major; Reservoir; Vector.

Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. kDNA nested PCR amplification (680 bp).
L. infantum in P. papatasi (Lane 1, 560 bp); L. major in P. papatasi and T. indica (Lanes 2 and 3); Positive control of L. major (Lane 4, 720 bp); Positive control of L. tropica (Lane 5); Negative control (Lane 6) and (M)100 bp molecular weight marker (Fermentase).
Figure 2.
Figure 2.. Promastigote and amastigote infection of wild caught Tatera indica after two month keeping in animal house.

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