Simultaneous recordings of glucose dependent electrical activity and ATP-regulated K(+)-currents in isolated mouse pancreatic beta-cells

FEBS Lett. 1990 Feb 12;261(1):187-90. doi: 10.1016/0014-5793(90)80667-8.


Membrane potential and membrane currents were recorded from single mouse pancreatic beta-cells using the perforated patch whole-cell recording technique at 30 degrees C. Single beta-cells maintained in primary tissue culture exhibited glucose-dependent electrical activity similar to that reported for freshly isolated intact islets. The resting input conductance (5.1 +/- 0.9 nS) was determined by ATP-regulated K+ (KATP) channels as it was blocked by 1 mM tolbutamide. 8 mM glucose decreased the input conductance by 80%. The input conductance at -70 mV was of a similar value during the plateau phase and during the silent phase of electrical activity in 8 mM glucose. This suggests that oscillations of KATP channel activity do not underlie the slow waves.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / pharmacology*
  • Animals
  • Cells, Cultured
  • Electric Conductivity
  • Glucose / pharmacology*
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / physiology*
  • Membrane Potentials / drug effects
  • Mice
  • Potassium Channels / drug effects
  • Potassium Channels / physiology*
  • Tolbutamide / pharmacology


  • Potassium Channels
  • Adenosine Triphosphate
  • Tolbutamide
  • Glucose