Distinct tRNA modifications in the thermo-acidophilic archaeon, Thermoplasma acidophilum

FEBS Lett. 2013 Nov 1;587(21):3575-80. doi: 10.1016/j.febslet.2013.09.021. Epub 2013 Sep 25.


Thermoplasma acidophilum is a thermo-acidophilic archaeon. We purified tRNA(Leu) (UAG) from T. acidophilum using a solid-phase DNA probe method and determined the RNA sequence after determining via nucleoside analysis and m(7)G-specific aniline cleavage because it has been reported that T. acidophilum tRNA contains m(7)G, which is generally not found in archaeal tRNAs. RNA sequencing and liquid chromatography-mass spectrometry revealed that the m(7)G modification exists at a novel position 49. Furthermore, we found several distinct modifications, which have not previously been found in archaeal tRNA, such as 4-thiouridine9, archaeosine13 and 5-carbamoylmethyuridine34. The related tRNA modification enzymes and their genes are discussed.

Keywords: 4-Thiouridine; 5-Carbamoylmethyuridine; 7-Methylguanine; Archaea; Archaeosine; tRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Archaeal Proteins / metabolism
  • Base Sequence
  • Guanosine / chemistry
  • Mass Spectrometry
  • Nucleic Acid Conformation
  • RNA, Transfer / chemistry*
  • Thermoplasma / genetics
  • Thermoplasma / metabolism*


  • Archaeal Proteins
  • Guanosine
  • RNA, Transfer