Parallel measurements of reaction kinetics using ultralow-volumes

Lab Chip. 2013 Nov 21;13(22):4326-30. doi: 10.1039/c3lc50768h.

Abstract

We present a new platform for the production and manipulation of microfluidic droplets in view of measuring the evolution of a chemical reaction. Contrary to existing approaches, our device uses gradients of confinement to produce a single drop on demand and guide it to a pre-determined location. In this way, two nanoliter drops containing different reagents can be placed in contact and merged together, in order to trigger a chemical reaction. The reaction rate is extracted from an analysis of the observed reaction-diffusion front. We show that the results obtained using this platform are in excellent agreement with stopped-flow measurements, while decreasing the sample consumption 5000 fold. We also show how the device operation can be parallelized in order to react an initial sample with a range of compounds or concentrations, on a single integrated chip. This integrated chip thus further reduces sample consumption while reducing the time required for the experimental runs from hours to minutes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,6-Dichloroindophenol / chemistry
  • Ascorbic Acid / chemistry
  • Diffusion
  • Kinetics
  • Microfluidic Analytical Techniques / instrumentation*
  • Oils / chemistry
  • Polyethylene Glycols / chemistry

Substances

  • Oils
  • Polyethylene Glycols
  • 2,6-Dichloroindophenol
  • Ascorbic Acid