Rescuing recombinant proteins by sequestration into the P22 VLP

Chem Commun (Camb). 2013 Nov 14;49(88):10412-4. doi: 10.1039/c3cc46517a.

Abstract

Here we report the use of a self-assembling protein cage to sequester and solubilize recombinant proteins which are usually trafficked to insoluble inclusion bodies. Our results suggest that protein cages can be used as novel vehicles to rescue and produce soluble proteins that are otherwise difficult to obtain using conventional methods.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteriophage P22 / chemistry*
  • Capsid / chemistry
  • Genetic Engineering / methods*
  • Inclusion Bodies / metabolism
  • Models, Molecular
  • Protein Conformation
  • Recombinant Proteins / chemistry*
  • Recombinant Proteins / genetics*
  • Solubility

Substances

  • Recombinant Proteins