Abstract
Cold-sensitive mutations in the SPB genes (spb1-spb7) of Saccharomyces cerevisiae suppress the inhibition of translation initiation resulting from deletion of the poly(A)-binding protein gene (PAB1). The SPB4 protein belongs to a family of adenosine triphosphate (ATP)-dependent RNA helicases. The aberrant production of 25S ribosomal RNA (rRNA) occurring in spb4-1 mutants or the deletion of SPB2 (RPL46) permits the deletion of PAB1. These data suggest that mutations affecting different steps of 60S subunit formation can allow PAB-independent translation, and they indicate that further characterization of the spb mutations could lend insight into the biogenesis of the ribosome.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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DEAD-box RNA Helicases
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Molecular Sequence Data
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Mutation
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Poly(A)-Binding Proteins
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Protein Biosynthesis*
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RNA Nucleotidyltransferases / genetics
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RNA Nucleotidyltransferases / metabolism*
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RNA Processing, Post-Transcriptional
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RNA, Fungal / genetics
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RNA, Fungal / metabolism
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RNA, Ribosomal / genetics
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RNA, Ribosomal / metabolism*
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism*
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Ribosomes / metabolism*
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Nucleic Acid
Substances
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Carrier Proteins
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Poly(A)-Binding Proteins
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RNA, Fungal
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RNA, Ribosomal
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Ribosomal Proteins
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Saccharomyces cerevisiae Proteins
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RNA Nucleotidyltransferases
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SPB4 protein, S cerevisiae
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DEAD-box RNA Helicases