Application of monoclonal antibodies and genetically-engineered hybrid B-subunit proteins to the analysis of the cholera/coli enterotoxin family

Dev Biol Stand. 1985:59:51-62.

Abstract

Single amino acid substitutions, introduced by genetic engineering, significantly modify the behavior of the B-subunits of the cholera/coli enterotoxin family in SDS-PAGE and also markedly affect the reactivity of the proteins with mouse hybridoma-derived monoclonal antibodies raised against H-LT. The results indicate that single amino acids play an important role in defining epitopes in these proteins.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / immunology*
  • Bacterial Toxins / analysis
  • Bacterial Toxins / immunology*
  • Cholera Toxin / analysis
  • Cholera Toxin / immunology*
  • Enterotoxins / analysis
  • Enterotoxins / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / analysis
  • Escherichia coli Proteins*
  • Genetic Engineering*

Substances

  • Antibodies, Monoclonal
  • Bacterial Toxins
  • Enterotoxins
  • Epitopes
  • Escherichia coli Proteins
  • Cholera Toxin
  • heat-labile enterotoxin, E coli