Abstract
Single amino acid substitutions, introduced by genetic engineering, significantly modify the behavior of the B-subunits of the cholera/coli enterotoxin family in SDS-PAGE and also markedly affect the reactivity of the proteins with mouse hybridoma-derived monoclonal antibodies raised against H-LT. The results indicate that single amino acids play an important role in defining epitopes in these proteins.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal / immunology*
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Bacterial Toxins / analysis
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Bacterial Toxins / immunology*
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Cholera Toxin / analysis
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Cholera Toxin / immunology*
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Enterotoxins / analysis
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Enterotoxins / immunology*
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Enzyme-Linked Immunosorbent Assay
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Epitopes / analysis
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Escherichia coli Proteins*
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Genetic Engineering*
Substances
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Antibodies, Monoclonal
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Bacterial Toxins
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Enterotoxins
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Epitopes
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Escherichia coli Proteins
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Cholera Toxin
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heat-labile enterotoxin, E coli