Snapshot of a DNA polymerase while incorporating two consecutive C5-modified nucleotides

J Am Chem Soc. 2013 Oct 23;135(42):15667-9. doi: 10.1021/ja405346s. Epub 2013 Oct 9.


Functional nucleotides are important in many cutting-edge biomolecular techniques. Often several modified nucleotides have to be incorporated consecutively. This structural study of KlenTaq DNA polymerase, a truncated form of Thermus aquaticus DNA polymerase, gives first insights how multiple modifications are processed by a DNA polymerase and, therefore, contribute to the understanding of these enzymes in their interplay with artificial substrates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Models, Molecular
  • Molecular Structure
  • Nucleotides / chemistry*
  • Nucleotides / metabolism
  • Taq Polymerase / chemistry*
  • Taq Polymerase / metabolism
  • Thermus / enzymology*


  • Nucleotides
  • Taq Polymerase