Bioremediation assessment of diesel-biodiesel-contaminated soil using an alternative bioaugmentation strategy

Environ Sci Pollut Res Int. 2014 Feb;21(4):2592-602. doi: 10.1007/s11356-013-2139-2. Epub 2013 Oct 5.


This study investigated the effectiveness of successive bioaugmentation, conventional bioaugmentation, and biostimulation of biodegradation of B10 in soil. In addition, the structure of the soil microbial community was assessed by polymerase chain reaction-denaturing gradient gel electrophoresis. The consortium was inoculated on the initial and the 11th day of incubation for successive bioaugmentation and only on the initial day for bioaugmentation and conventional bioaugmentation. The experiment was conducted for 32 days. The microbial consortium was identified based on sequencing of 16S rRNA gene and consisted as Pseudomonas aeruginosa, Achromobacter xylosoxidans, and Ochrobactrum intermedium. Nutrient introduction (biostimulation) promoted a positive effect on microbial populations. The results indicate that the edaphic community structure and dynamics were different according to the treatments employed. CO2 evolution demonstrated no significant difference in soil microbial activity between biostimulation and bioaugmentation treatments. The total petroleum hydrocarbon (TPH) analysis indicated a biodegradation level of 35.7 and 32.2 % for the biostimulation and successive bioaugmentation treatments, respectively. Successive bioaugmentation displayed positive effects on biodegradation, with a substantial reduction in TPH levels.

MeSH terms

  • Achromobacter denitrificans / isolation & purification
  • Achromobacter denitrificans / physiology*
  • Biodegradation, Environmental
  • Biofuels*
  • Brucellaceae / isolation & purification
  • Brucellaceae / physiology*
  • Carbon Dioxide / metabolism
  • DNA, Bacterial / genetics
  • Denaturing Gradient Gel Electrophoresis
  • Gasoline*
  • Hydrocarbons / metabolism*
  • Microbial Consortia / physiology
  • Polymerase Chain Reaction
  • Pseudomonas aeruginosa / isolation & purification
  • Pseudomonas aeruginosa / physiology*
  • RNA, Ribosomal, 16S / genetics
  • Soil Microbiology
  • Soil Pollutants / metabolism*


  • Biofuels
  • DNA, Bacterial
  • Gasoline
  • Hydrocarbons
  • RNA, Ribosomal, 16S
  • Soil Pollutants
  • Carbon Dioxide