Multiple signaling pathways coordinate to induce a threshold response in a chordate embryo

PLoS Genet. 2013;9(10):e1003818. doi: 10.1371/journal.pgen.1003818. Epub 2013 Oct 3.

Abstract

In animal development, secreted signaling molecules evoke all-or-none threshold responses of target gene transcription to specify cell fates. In the chordate Ciona intestinalis, the neural markers Otx and Nodal are induced at early embryonic stages by Fgf9/16/20 signaling. Here we show that three additional signaling molecules act negatively to generate a sharp expression boundary for neural genes. EphrinA signaling antagonizes FGF signaling by inhibiting ERK phosphorylation more strongly in epidermal cells than in neural cells, which accentuates differences in the strength of ERK activation. However, even weakly activated ERK activates Otx and Nodal transcription occasionally, probably because of the inherently stochastic nature of signal transduction processes and binding of transcription factors to target sequences. This occasional and undesirable activation of neural genes by weak residual ERK activity is directly repressed by Smad transcription factors activated by Admp and Gdf1/3-like signaling, further sharpening the differential responses of cells to FGF signaling. Thus, these signaling pathways coordinate to evoke a threshold response that delineates a sharp expression boundary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Patterning
  • Cell Differentiation / genetics
  • Chordata / growth & development
  • Ciona intestinalis / genetics
  • Ciona intestinalis / growth & development
  • Embryo, Nonmammalian*
  • Embryonic Development / genetics*
  • Ephrins / genetics*
  • Ephrins / metabolism
  • Fibroblast Growth Factors / genetics
  • Gene Expression Regulation, Developmental
  • Otx Transcription Factors / genetics
  • Phosphorylation
  • Signal Transduction / genetics*
  • Transcription, Genetic

Substances

  • Ephrins
  • Otx Transcription Factors
  • Fibroblast Growth Factors

Grant support

This research was supported by Grants-in-Aid from JSPS (http://www.jsps.go.jp), Japan, to YS (21671004). NO was supported by a predoctral fellowship (DC2) with a grant from JSPS (24-360). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.