One-pot, single-step deracemization of 2-hydroxyacids by tandem biocatalytic oxidation and reduction

Ya-Ping Xue; Yu-Guo Zheng; Ya-Qin Zhang; Jing-Lei Sun; Zhi-Qiang Liu; Yin-Chu Shen

doi:10.1039/c3cc46240d

One-pot, single-step deracemization of 2-hydroxyacids by tandem biocatalytic oxidation and reduction

Chem Commun (Camb). 2013 Nov 25;49(91):10706-8. doi: 10.1039/c3cc46240d.

Authors

Ya-Ping Xue¹, Yu-Guo Zheng, Ya-Qin Zhang, Jing-Lei Sun, Zhi-Qiang Liu, Yin-Chu Shen

Affiliation

¹ Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China. zhengyg@zjut.edu.cn.

PMID: 24100587
DOI: 10.1039/c3cc46240d

Abstract

A facile and efficient one-pot, single-step method for deracemizing a broad range of 2-hydroxyacids to (R)-2-hydroxyacids was established by combination of resting cells of an (S)-hydroxyacid dehydrogenase-producing microorganism and an (R)-ketoacid reductase-producing microorganism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Oxidoreductases / metabolism*
Biocatalysis*
Hydroxy Acids / chemistry*
Oxidation-Reduction
Pseudomonas aeruginosa / enzymology
Saccharomyces cerevisiae / enzymology
Stereoisomerism
Substrate Specificity

Substances

Hydroxy Acids
Alcohol Oxidoreductases