Intracerebral hematomas disappear on T2*-weighted images during normobaric oxygen therapy

Stroke. 2013 Dec;44(12):3482-9. doi: 10.1161/STROKEAHA.113.002045. Epub 2013 Oct 8.


Background and purpose: The aim of the present study was to investigate the effects of normobaric oxygen (NBO) therapy on T2*-weighted images of intracranial hemorrhages (ICHs).

Methods: Two common models of ICH were performed in mice, and longitudinal T2*-weighted images of the hematomas were acquired under normoxia or NBO. The effects of NBO were also investigated on perfusion-weighted imaging, susceptibility-weighted imaging, and molecular imaging of vascular cell adhesion molecule-1 after ICH. Last, we performed neurological testing, including neuroscore, actimetry, and gait analysis (Catwalk), to study the influence of NBO on neurological outcome of mice presenting ICH.

Results: Our results demonstrated that NBO, even during a short period of time, dramatically reduces the sensitivity of T2*-weighted imaging to detect ICH. Moreover, we provide evidence that the disappearance of ICH on T2*-weighted imaging could be used to improve accuracy of perfusion-weighted imaging and to allow molecular imaging after ICH. Importantly, a 30-minute NBO preparation 24 hours after ICH onset does not influence neurological outcome.

Conclusions: We provide an experimental demonstration that NBO significantly affects T2*-weighted imaging in ICH. Although this phenomenon could lead to inaccurate assessment of ICH volume, it could also be safely used to allow perfusion-weighted imaging and molecular imaging.

Keywords: behavior therapy; cerebral hemorrhage; magnetic resonance imaging; molecular imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / pathology*
  • Intracranial Hemorrhages / blood
  • Intracranial Hemorrhages / pathology*
  • Intracranial Hemorrhages / therapy
  • Magnetic Resonance Imaging
  • Mice
  • Oxygen / blood
  • Oxygen Inhalation Therapy*
  • Treatment Outcome


  • Oxygen