Live pigs produced from genome edited zygotes

Sci Rep. 2013 Oct 10:3:2847. doi: 10.1038/srep02847.


Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Animals, Genetically Modified / genetics*
  • Animals, Genetically Modified / growth & development
  • Base Sequence
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / metabolism
  • Endonucleases / metabolism
  • Female
  • Fertilization in Vitro*
  • Gene Knockout Techniques
  • Genetic Engineering*
  • Genome*
  • Homozygote
  • Molecular Sequence Data
  • Nuclear Transfer Techniques
  • RNA Editing / genetics*
  • Sequence Homology, Nucleic Acid
  • Swine
  • Zinc Fingers / genetics*
  • Zygote / cytology*
  • Zygote / metabolism


  • Endonucleases