Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vicina Roger)

Int J Syst Evol Microbiol. 2014 Feb;64(Pt 2):495-500. doi: 10.1099/ijs.0.055863-0. Epub 2013 Oct 9.


A novel actinomycete, designated strain NEAU-ycm2(T), was isolated from edible Chinese black ants (Polyrhachis vicina Roger) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-ycm2(T) showed highest similarity to those of Micromonospora sonneratiae 274745(T) (99.12%), Micromonospora pattaloongensis TJ2-2(T) (98.85%), Micromonospora pisi GUI 15(T) (98.76%), Polymorphospora rubra TT 97-42(T) (98.42%) and Micromonospora eburnea LK2-10(T) (98.21%). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-ycm2(T) is a member of the genus Micromonospora and supported the close phylogenetic relationship to M. sonneratiae 274745(T), M. pattaloongensis JCM 12833(T) and M. pisi GUI 15(T). Furthermore, a combination of DNA-DNA hybridization and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. Therefore, it is proposed that NEAU-ycm2(T) represents a novel species of the genus of Micromonospora, for which the name Micromonospora polyrhachis sp. nov. is proposed. The type strain is NEAU-ycm2(T) ( = CGMCC 4.7100(T) = DSM 45886(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ants / microbiology*
  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Genes, Bacterial
  • Micromonospora / classification*
  • Micromonospora / genetics
  • Micromonospora / isolation & purification
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vitamin K 2 / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2

Associated data

  • GENBANK/KC139400
  • GENBANK/KC977996