Steady-state fluorescence polarization/anisotropy for the study of protein interactions

Methods Mol Biol. 2014;1076:29-42. doi: 10.1007/978-1-62703-649-8_2.


Fluorescence methods are often employed for the characterization of molecular interactions. In particular, polarization/anisotropy studies are widely utilized in the life sciences as they allow quantification of protein interactions in the micro- and nanomolar concentration range. Herein we shall briefly describe the theoretical aspects of polarization/anisotropy and outline an experiment for determination of the dissociation constant for a protein-ligand complex.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anisotropy
  • Fluorescence Polarization / methods*
  • Fluorescent Dyes / chemistry*
  • Ligands
  • Protein Interaction Maps
  • Proteins / chemistry*
  • Spectrometry, Fluorescence


  • Fluorescent Dyes
  • Ligands
  • Proteins