Frequency domain fluorometry: theory and application

Methods Mol Biol. 2014;1076:77-95. doi: 10.1007/978-1-62703-649-8_5.


Frequency domain fluorometry is a widely utilized tool in the physical, chemical, and biological sciences. This chapter focuses on the theory of the method and the practical aspects required to carry out intensity decay, i.e., lifetime measurements on a modern frequency domain fluorometer. Several chemical/biological systems are utilized to illustrate data acquisition protocols. Data analysis procedures and methodologies are also discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescence*
  • Fluorometry / methods*
  • Models, Molecular
  • Molecular Biology / methods*