Abstract
Recent advances in understanding how actinomycin binds to DNA have suggested its mechanism of action. Actinomycin binds to a premelted DNA conformation present within the transcriptional complex. This immobilizes the complex, interfering with the elongation of growing RNA chains. The model has a number of implications for understanding RNA synthesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Nucleolus / metabolism
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DNA-Directed RNA Polymerases / metabolism
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Dactinomycin / pharmacology*
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Microscopy, Electron
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Models, Molecular
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Molecular Conformation
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Nucleic Acid Conformation
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Nucleic Acid Denaturation
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Transcription, Genetic / drug effects*
Substances
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Dactinomycin
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DNA-Directed RNA Polymerases