Mucosubstance histochemistry of the normal mucosa and epithelial neoplasms of the large intestine

Acta Pathol Jpn. 1985 Mar;35(2):281-97. doi: 10.1111/j.1440-1827.1985.tb00574.x.


Histochemical properties of mucosubstances in normal mucosa and neoplasms of 100 operated cases with colonic carcinoma were investigated by using several new methods, such as paradoxical concanavalin A (con A) staining and modified PAS reactions for sialic acids. Acidity of goblet cell-type mucin (GCM) of the normal mucosa varied with the depth of the crypt, as well as with different segments of the large intestine, whereas surface coat-type mucin (SCM) mainly consisted of sulfomucin throughout the large intestine. In addition, the PAS reactivity revealing the presence of O-acetylated sialic acid and the labile class III con A reactivity were demonstrated as hallmarks characterizing the colonic GCM. In carcinoma tissues, sialomucin was more abundant than in the normal mucosa. Goblet-type tumor cells were found in 59 cases. Moreover, O-acetylated sialic acid and the labile class III con A reactivity persisted in GCM of the goblet-type tumor cells. GCM of the adjacent mucosa of the neoplasms showed a decrease in sulfomucin in 95 cases and a marked increase in the labile class III con A reactivity in 97 cases, while the histochemical properties of SCM in this region remained unchanged.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Colon / analysis*
  • Colonic Neoplasms / analysis*
  • Colonic Polyps / analysis
  • Epithelium / analysis
  • Female
  • Humans
  • Intestinal Mucosa / analysis*
  • Male
  • Middle Aged
  • Mucins / analysis*
  • Periodic Acid-Schiff Reaction
  • Rats
  • Sialic Acids / analysis
  • Staining and Labeling


  • Mucins
  • Sialic Acids
  • sulfomucin