Evaluation of different culture methods for the diagnosis of peritonitis in patients on continuous ambulatory peritoneal dialysis

Clin Microbiol Infect. 2014 May;20(5):O294-6. doi: 10.1111/1469-0691.12402. Epub 2013 Nov 11.


A total of 170 continuous ambulatory peritoneal dialysis (CAPD) fluids were processed by various culture methods, including direct inoculation of the centrifuged sediment, inoculation into automated blood culture bottles, water lysis, Tween-80 incorporated blood agar, and Triton-X treatment of the specimen. Of 170 CAPD fluids, 127 showed the growth of bacteria/fungi. Sixty-three fluids showed growth by all methods, the water lysis alone detected 24 additional positive cultures, while Tween-80 blood agar and Triton-X yielded 30 additional positive cultures. A combination of water lysis, Tween-80 blood agar and Triton-X treatment of the CAPD fluid is recommended for diagnosis of CAPD peritonitis in resource-limited settings.

Keywords: BACTEC; CAPD; Triton-X; Tween-80; water lysis.

Publication types

  • Evaluation Study

MeSH terms

  • Ascites / microbiology*
  • Bacterial Infections / diagnosis*
  • Bacterial Infections / microbiology
  • Bacteriological Techniques
  • Blood
  • Culture Media
  • Humans
  • Peritoneal Dialysis, Continuous Ambulatory / adverse effects*
  • Peritonitis / diagnosis*
  • Peritonitis / microbiology
  • Specimen Handling / methods*


  • Culture Media