The receptor-destroying enzyme of influenza C virus is neuraminate-O-acetylesterase

EMBO J. 1985 Jun;4(6):1503-6.

Abstract

The nature of the receptor-destroying enzyme (RDE) of influenza C virus has been elucidated by analyzing its effect on the haemagglutination inhibitors rat alpha 1-macroglobulin (RMG) and bovine submandibulary mucin (BSM), respectively. The inhibitory activity of both compounds is abolished by incubation with influenza C virus. After inactivation, RMG and BSM were found to contain reduced amounts of N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) and increased amounts of N-acetylneuraminic acid (Neu5Ac). H.p.l.c. analysis revealed that purified Neu5,9Ac2 is converted to Neu5Ac by incubation with influenza C virus. These results demonstrate that RDE of influenza C virus is neuraminate-O-acetylesterase [N-acyl-9(4)-O-acetylneuraminate O-acetylhydrolase (EC 3.1.1.53)]. The data also indicate that haemagglutination-inhibition (HI) by RMG and BSM and most likely virus attachment to cell surfaces involves binding of influenza C virus to Neu5,9Ac2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylesterase / physiology*
  • Animals
  • Carboxylic Ester Hydrolases / analysis*
  • Cattle
  • Mucins / metabolism
  • Neuraminic Acids / metabolism*
  • Neuraminidase / physiology
  • Orthomyxoviridae / enzymology*
  • Receptors, Virus / metabolism*
  • alpha-Macroglobulins / metabolism

Substances

  • Mucins
  • Neuraminic Acids
  • Receptors, Virus
  • alpha-Macroglobulins
  • Carboxylic Ester Hydrolases
  • Acetylesterase
  • sialate O-acetylesterase
  • Neuraminidase