Abstract
Giardia lamblia is a protozoan parasite that is found worldwide and has both medical and veterinary importance. We applied the transcription start sequence (TSS-seq) and RNA sequence (RNA-seq) techniques to study the transcriptome of the assemblage A WB strain trophozoite. We identified 8000 transcription regions (TR) with significant transcription. Of these regions, 1881 TRs were more than 500 nucleotides upstream of an annotated ORF. Combining both techniques helped us to identify 24 ORFs that should be re-annotated and 60 new ORFs. From the 8000 TRs, we were able to identify an AT-rich consensus that includes the transcription initiation site. It is possible that transcription that was previously thought to be bidirectional is actually unidirectional.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Gene Expression Profiling / methods*
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Genes, Protozoan / genetics
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Giardia lamblia / genetics*
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Models, Genetic
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Open Reading Frames / genetics
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RNA, Protozoan / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, RNA / methods*
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Transcription Initiation Site*
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Transcription, Genetic
Grants and funding
Mohammed E. M. Tolba was supported by The Tokyo Biochemical Research Foundation (TBRF) for postdoctoral fellowship. This work was in part supported by KAKENHI (Grant-in-Aid for Scientific Research) in the Priority Area of “Genome Science” from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.