Parvalbumin is overexpressed in the late phase of pharmacological preconditioning in skeletal muscle

Can J Physiol Pharmacol. 2013 Nov;91(11):966-72. doi: 10.1139/cjpp-2013-0113. Epub 2013 Jul 17.


Pharmacological preconditioning (PPC) with mitochondrial ATP-sensitive K(+) channel openers such as diazoxide, provides protection against ischemia in cardiac muscle, skeletal muscle, and other tissues. Effects on Ca(2+) homeostasis during the late phase of PPC have been described in cardiomyocytes, but no information is available regarding intracellular Ca(2+) changes in skeletal muscle fibers during late PPC. Intracellular Ca(2+) signals were measured in single fibers of adult mouse skeletal muscle, with fluorescent probes, 48 h after the administration of diazoxide. Parvalbumin levels in the myofibers were quantitated by Western blot. Diazoxide induction of late PPC was confirmed by partial protection of muscles from peroxide-induced damage. Late PPC was associated with a significant decrease in the duration of Ca(2+) signals during single twitches and tetanus with no changes in peak values. This effect was prevented by the reactive oxygen species (ROS) scavenger tiron. Late PPC was accompanied by a 30% increase in parvalbumin levels, and this effect was also blocked by tiron. Our data show, for the first time, a role of parvalbumin in late PPC in skeletal muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Aniline Compounds
  • Animals
  • Blotting, Western
  • Calcium / metabolism
  • Calcium Signaling / drug effects
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2 / antagonists & inhibitors
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Diazoxide / pharmacology
  • Electric Stimulation
  • Fluorescent Dyes
  • Homeostasis / drug effects
  • KATP Channels / agonists*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Muscle Fibers, Skeletal / drug effects
  • Muscle, Skeletal / drug effects*
  • Parvalbumins / biosynthesis*
  • Protein Kinase Inhibitors / pharmacology
  • Reactive Oxygen Species
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism
  • Xanthenes


  • Aniline Compounds
  • Fluorescent Dyes
  • KATP Channels
  • Parvalbumins
  • Protein Kinase Inhibitors
  • Reactive Oxygen Species
  • Xanthenes
  • Fluo-3
  • Cyclic AMP-Dependent Protein Kinases
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Diazoxide
  • Calcium