Protection against a fungal pathogen conferred by the aphid facultative endosymbionts Rickettsia and Spiroplasma is expressed in multiple host genotypes and species and is not influenced by co-infection with another symbiont

J Evol Biol. 2013 Dec;26(12):2654-61. doi: 10.1111/jeb.12260. Epub 2013 Oct 7.

Abstract

Many insects harbour facultative endosymbiotic bacteria, often more than one type at a time. These symbionts can have major effects on their hosts' biology, which may be modulated by the presence of other symbiont species and by the host's genetic background. We investigated these effects by transferring two sets of facultative endosymbionts (one Hamiltonella and Rickettsia, the other Hamiltonella and Spiroplasma) from naturally double-infected pea aphid hosts into five novel host genotypes of two aphid species. The symbionts were transferred either together or separately. We then measured aphid fecundity and susceptibility to an entomopathogenic fungus. The pathogen-protective phenotype conferred by the symbionts Rickettsia and Spiroplasma varied among host genotypes, but was not influenced by co-infection with Hamiltonella. Fecundity varied across single and double infections and between symbiont types, aphid genotypes and species. Some host genotypes benefit from harbouring more than one symbiont type.

Keywords: Acyrthosiphon pisum; Pandora neoaphidis; Sitobion avenae; endosymbiosis; inclusive fitness; resistance; secondary symbiont.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphids / genetics
  • Aphids / microbiology*
  • Fungi / pathogenicity*
  • Genotype
  • Host-Pathogen Interactions*
  • Rickettsia / pathogenicity*
  • Spiroplasma / pathogenicity*
  • Symbiosis*