In this contribution we present a sensitive colorimetric bioactive paper fabricated to determine sialidase-related diseases like bacterial vaginosis (BV) in a one-step and dry format spot assay with fast response and good storage stability. The paper was prepared by three simple steps. The first step involves preparation of poly(ethyleneimine) (PEI) microcapsules, the second step is to incubate positively charged microcapsules in negatively charged 5-bromo-4-chloro-3-indolyl-a-D-N-acetylneuraminic acid (BCIN) solution, a color enhancer nitro blue tetrazolium (NBT), and in the third step, paper was fabricated by incorporating incubated microcapsules into paper pulp. This paper changes color from white to dark purple in the presence of sialidase in as little as 6min, and color could be enhanced with increased length of reaction time. In this reaction system, BCIN was the substrate for sialidase, NBT was the color enhancer, and PEI microcapsules acted as catalyst. The loading efficiency of BCIN was about 22.2%, and filtered BCIN solution could be reused for the next fabrication.
Keywords: Bioactive paper; Bioactive paper strip; Colorimetric biosensor; Microcapsules; Neuraminidase.
Copyright © 2013 Elsevier B.V. All rights reserved.