Zebrafish brain proteomics reveals central proteins involved in neurodegeneration

J Neurosci Res. 2014 Jan;92(1):104-15. doi: 10.1002/jnr.23297. Epub 2013 Oct 5.


Understanding the complex biology of the brain requires analyzing its structural and functional complexity at the protein level. The large-scale analysis of the brain proteome, coupled with characterization of central brain proteins, provides insight into fundamental brain processes and processes linked to neurodegenerative diseases. Here we provide a map of the zebrafish brain proteome by using two-dimensional gel electrophoresis (2DE), followed by the identification of 95 brain proteins using mass spectrometry (LC-ESI MS/MS). Our data show extensive phosphorylation of brain proteins but less prominent glycosylation. Furthermore, ~51% of the identified proteins are predicted to have one or more ubiquitination sites whereas ~90% are predicted to have one or more SUMOylation sites. Our findings provide a valuable proteome map of the zebrafish brain and associated posttranslational modifications demonstrating that zebrafish proteomic approaches can aid in our understanding of proteins central to important neuronal processes and those associated with neurodegenerative disorders.

Keywords: brain; neurodegeneration; proteomics; zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • Electrophoresis, Gel, Two-Dimensional
  • Glycosylation
  • Nerve Degeneration / genetics
  • Nerve Degeneration / metabolism*
  • Phosphorylation
  • Proteome / genetics
  • Proteome / metabolism*
  • Proteomics / methods
  • Tandem Mass Spectrometry
  • Zebrafish / genetics
  • Zebrafish / metabolism
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism*


  • Proteome
  • Zebrafish Proteins