Proteolytic characterisation in grass carp sausage inoculated with Lactobacillus plantarum and Pediococcus pentosaceus

Food Chem. 2014 Feb 15:145:840-4. doi: 10.1016/j.foodchem.2013.08.096. Epub 2013 Sep 5.

Abstract

The proteolysis in grass carp sausages inoculated with Lactobacillus plantarum ZY40 and Pediococcus pentosaceus GY23 was investigated. As fermentation progressed, sarcoplasmic and myofibrillar proteins in both sausages were obviously degraded, and the proteolytic process was more intense in sausages inoculated with P. pentosaceus GY23. The increases in α-amino nitrogen, trichloroacetic acid (TCA)-soluble peptides and free amino acids were also detected in both sausages. The differences in α-amino nitrogen content and free amino acids concentration were due to the activity of inoculated lactic acid bacteria, while endogenous enzymes contributed to the release of TCA-soluble peptides. Our findings indicate that lactic acid bacteria influence proteolytic characterisation in fermented fish sausage, with strain-dependent activity.

Keywords: Grass carp sausage; Lactobacillus plantarum; Pediococcus pentosaceus; Proteolysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / analysis
  • Amino Acids / metabolism
  • Animals
  • Carps*
  • China
  • Diet / ethnology
  • Dietary Proteins / analysis
  • Dietary Proteins / chemistry
  • Dietary Proteins / metabolism*
  • Fermentation
  • Fish Products / analysis*
  • Fish Products / microbiology
  • Fish Proteins / analysis
  • Fish Proteins / chemistry
  • Fish Proteins / metabolism*
  • Food Storage
  • Food, Preserved / analysis*
  • Food, Preserved / microbiology
  • Humans
  • Hydrogen-Ion Concentration
  • Lactobacillus plantarum / growth & development
  • Lactobacillus plantarum / isolation & purification
  • Lactobacillus plantarum / metabolism*
  • Muscle Proteins / analysis
  • Muscle Proteins / chemistry
  • Muscle Proteins / metabolism
  • Pediococcus / growth & development
  • Pediococcus / isolation & purification
  • Pediococcus / metabolism*
  • Peptide Fragments / analysis
  • Peptide Fragments / metabolism
  • Proteolysis
  • Sodium Chloride, Dietary / analysis
  • Species Specificity

Substances

  • Amino Acids
  • Dietary Proteins
  • Fish Proteins
  • Muscle Proteins
  • Peptide Fragments
  • Sodium Chloride, Dietary