Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

Gene. 1985;35(3):279-87. doi: 10.1016/0378-1119(85)90006-x.


A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZ alpha expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / immunology
  • Antigens, Viral / genetics*
  • Cloning, Molecular
  • Epitopes
  • Escherichia coli / genetics
  • Genes, Viral
  • Glycoproteins / genetics
  • Molecular Weight
  • Simplexvirus / genetics*
  • Simplexvirus / immunology
  • Viral Proteins / genetics*
  • Viral Vaccines / immunology


  • Antibodies, Viral
  • Antigens, Viral
  • Epitopes
  • Glycoproteins
  • Viral Proteins
  • Viral Vaccines

Associated data

  • GENBANK/M11278
  • GENBANK/M11347