A comparative proteomic analysis of Pinellia ternata leaves exposed to heat stress

Int J Mol Sci. 2013 Oct 15;14(10):20614-34. doi: 10.3390/ijms141020614.


Pinellia ternata is an important traditional Chinese medicinal plant. The growth of P. ternata is sensitive to high temperatures. To gain a better understanding of heat stress responses in P. ternata, we performed a comparative proteomic analysis. P. ternata seedlings were subjected to a temperature of 38 °C and samples were collected 24 h after treatment. Increased relative ion leakage and lipid peroxidation suggested that oxidative stress was frequently generated in rice leaves exposed to high temperature. Two-dimensional electrophoresis (2-DE) was used to analyze heat-responsive proteins. More than 600 protein spots were reproducibly detected on each gel; of these spots, 20 were up-regulated, and 7 were down-regulated. A total of 24 proteins and protein species were successfully identified by MALDI-TOF/TOF MS. These proteins and protein species were found to be primarily small heat shock proteins (58%) as well as proteins involved in RNA processing (17%), photosynthesis (13%), chlorophyll biosynthetic processes (4%), protein degradation (4%) and defense (4%). Using 2-DE Western blot analysis, we confirmed the identities of the cytosolic class II small heat shock protein (sHSPs-CII) identified by MS. The expression levels of four different proteins [cytosolic class I small heat shock protein (sHSPs-CI), sHSPs-CII, mitochondrial small heat shock protein (sHSPs-MIT), glycine-rich RNA-binding protein (GRP)] were analyzed at the transcriptional level by quantitative real-time PCR. The mRNA levels of three sHSPs correlated with the corresponding protein levels. However, GRP was down-regulated at the beginning of heat stress but then increased substantially to reach a peak after 24 h of heat stress. Our study provides valuable new insight into the responses of P. ternata to heat stress.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlorophyll / biosynthesis
  • Down-Regulation / genetics
  • Gene Expression / genetics
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism
  • Hot Temperature
  • Lipid Peroxidation / genetics
  • Oxidative Stress / genetics
  • Photosynthesis / genetics
  • Pinellia / genetics*
  • Pinellia / metabolism*
  • Plant Leaves / genetics*
  • Plant Leaves / metabolism*
  • Proteolysis
  • Proteome / genetics*
  • Proteome / metabolism*
  • Proteomics / methods
  • RNA Processing, Post-Transcriptional / genetics
  • Stress, Physiological / genetics*
  • Up-Regulation / genetics


  • Heat-Shock Proteins
  • Proteome
  • Chlorophyll