Toxicity of perfluorooctanoic acid to Pseudomonas putida in the aquatic environment

Abstract

Perfluorinated compounds are potential persistent organic pollutants which attracted much concerns in recent years. Thus relevant toxicity data of perfluorooctanoic acid (PFOA) are vitally important for identification of possible risk in the aquatic environment. In the present study, the acute toxic effect of PFOA in the absence and presence of either chromium (III) or tetra butyl ammonium (TBA) towards Pseudomonas putida in the aquatic environment was investigated by microcalorimetry. The thermokinetic parameters including growth rate constant (k), inhibitory ratio, and half inhibitory concentration, were calculated and compared using the data obtained from the power-time curves. Our work revealed the toxicity of PFOA under three experimental conditions in a descending sequence: PFOA, PFOA+Cr(3+), and PFOA+TBA. The results highlighted that the presence of un-ionized NH3 in the test solutions could not be a potential significant contributor to the observed toxicity of PFOA. In addition, PFOA interacted antagonistically with Cr(3+) and TBA. TBA was found to substantially enhance the surface pressure of PFOA which could be related with the toxicity of PFOA. The higher surface pressure caused for the reduction in toxicity. Thus the results highlighted the potential toxicological risk associated with this surfactant in the aquatic ecosystems.

Keywords: Acute toxicity; Antagonism; Microcalorimetry; PFOA; Surface pressure.