Treatment with the reactive oxygen species scavenger EUK-207 reduces lung damage and increases survival during 1918 influenza virus infection in mice

Free Radic Biol Med. 2014 Feb;67:235-47. doi: 10.1016/j.freeradbiomed.2013.10.014. Epub 2013 Oct 17.

Abstract

The 1918 influenza pandemic caused over 40 million deaths worldwide, with 675,000 deaths in the United States alone. Studies in several experimental animal models showed that 1918 influenza virus infection resulted in severe lung pathology associated with dysregulated immune and cell death responses. To determine if reactive oxygen species produced by host inflammatory responses play a central role in promoting severity of lung pathology, we treated 1918 influenza virus-infected mice with the catalytic catalase/superoxide dismutase mimetic, salen-manganese complex EUK-207 beginning 3 days postinfection. Postexposure treatment of mice infected with a lethal dose of the 1918 influenza virus with EUK-207 resulted in significantly increased survival and reduced lung pathology without a reduction in viral titers. In vitro studies also showed that EUK-207 treatment did not affect 1918 influenza viral replication. Immunohistochemical analysis showed a reduction in the detection of the apoptosis marker cleaved caspase-3 and the oxidative stress marker 8-oxo-2'-deoxyguanosine in lungs of EUK-207-treated animals compared to vehicle controls. High-throughput sequencing and RNA expression microarray analysis revealed that treatment resulted in decreased expression of inflammatory response genes and increased lung metabolic and repair responses. These results directly demonstrate that 1918 influenza virus infection leads to an immunopathogenic immune response with excessive inflammatory and cell death responses that can be limited by treatment with the catalytic antioxidant EUK-207.

Keywords: Antioxidants; Free radicals; Immune response; Influenza; Pathogenesis; Reactive oxygen species; Virus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Animals
  • Biomarkers / metabolism
  • Caspase 3 / genetics
  • Caspase 3 / metabolism
  • DNA Repair
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / metabolism
  • Dogs
  • Female
  • Free Radical Scavengers / pharmacology*
  • Gene Expression
  • Humans
  • Inflammation / drug therapy
  • Inflammation / metabolism
  • Inflammation / mortality
  • Inflammation / virology
  • Influenza A Virus, H1N1 Subtype / pathogenicity
  • Influenza A Virus, H1N1 Subtype / physiology*
  • Influenza Pandemic, 1918-1919*
  • Lung / drug effects
  • Lung / metabolism
  • Lung / pathology
  • Madin Darby Canine Kidney Cells
  • Mice
  • Mice, Inbred BALB C
  • Organometallic Compounds / pharmacology*
  • Orthomyxoviridae Infections / drug therapy*
  • Orthomyxoviridae Infections / metabolism
  • Orthomyxoviridae Infections / mortality
  • Orthomyxoviridae Infections / virology
  • Reactive Oxygen Species / antagonists & inhibitors*
  • Reactive Oxygen Species / metabolism
  • Survival Analysis
  • Viral Load
  • Virus Replication

Substances

  • Biomarkers
  • EUK 207
  • Free Radical Scavengers
  • Organometallic Compounds
  • Reactive Oxygen Species
  • 8-Hydroxy-2'-Deoxyguanosine
  • Caspase 3
  • Deoxyguanosine