Insulin-specific T cell hybridomas derived from (H-2b x H-2k)F1 mice preferably employ F1-unique restriction elements for antigen recognition

Eur J Immunol. 1985 Oct;15(10):1048-54. doi: 10.1002/eji.1830151017.


T cell hybridomas of (B10 X B10.BR)F1 genotype with reactivity to bovine insulin (BI) were established to analyze the restriction and antigen fine specificity of (H-2b X H-2k)F1 T cells towards BI. Our data indicate a focusing of the response on two epitopes on the insulin molecule, the A chain loop determinant comprising amino acids A8 and A10, as well as the glutamic acid residue in position 4 of the A chain. Both were recognized either separately or in conjunction. Unexpectedly, the T cell hybridomas exhibited a marked preference for recognizing insulin in the context of F1-unique restriction elements of Ab alpha Ak beta type rather than parental high-responder I-Ab molecules. Analysis of the response of primed lymph node T cells of (B10 X B10.BR)F1 mice towards BI corroborated the finding of a preponderant corecognition of F1-unique I-A molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Epitopes
  • H-2 Antigens / immunology
  • Heterozygote
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / immunology*
  • Hybridomas / immunology*
  • Insulin / immunology*
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred Strains
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocytes / immunology*


  • Epitopes
  • H-2 Antigens
  • Histocompatibility Antigens Class II
  • Insulin
  • Receptors, Antigen, T-Cell