Panax notoginseng attenuates experimental colitis in the azoxymethane/dextran sulfate sodium mouse model

Phytother Res. 2014 Jun;28(6):892-8. doi: 10.1002/ptr.5066. Epub 2013 Oct 21.

Abstract

Patients suffering from inflammatory bowel disease are at a high risk of developing colorectal cancer. To assess the anticancer potential of botanicals, in this study, we evaluated the effects of Panax notoginseng on azoxymethane/dextran sulfate sodium (DSS)-induced colitis. One week after A/J mice received azoxymethane, the animals received DSS for 8 days or were supplemented with P. notoginseng extract, at 30 or 90 mg/kg. DSS-induced colitis was scored with the disease activity index. The severity of the inflammatory lesions was evaluated by a colon tissue histological assessment. The expression of inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) were also explored. We observed that the effects of P. notoginseng on the reduction of colon inflammation, expressed in disease activity index score, were in a dose-related manner (p < 0.01). P. notoginseng inhibited the reduction of the colon length and the loss of bodyweight in dose-related manner (all p < 0.05). The histological assessment of the colitis and inflammatory-related immunohistochemical data also supported the pharmacological observations. Our data suggest that P. notoginseng is a promising candidate in preventing and treating colitis and inflammation-associated colon carcinogenesis.

Keywords: AOM/DSS model; Panax notoginseng; colitis; colorectal carcinogenesis; inflammatory bowel disease; notoginseng.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Azoxymethane
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colitis / pathology
  • Colon / drug effects
  • Colon / pathology
  • Cyclooxygenase 2 / metabolism
  • Dextran Sulfate
  • Disease Models, Animal
  • Male
  • Mice
  • Nitric Oxide Synthase Type II / metabolism
  • Panax notoginseng / chemistry*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Saponins / chemistry

Substances

  • Plant Extracts
  • Saponins
  • Dextran Sulfate
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
  • Ptgs2 protein, mouse
  • Cyclooxygenase 2
  • Azoxymethane