Contrasting of biological samples for X-ray synchrotron microtomography

Bull Exp Biol Med. 2013 Aug;155(4):413-6. doi: 10.1007/s10517-013-2165-6.


The method of contrasting with iodine ions was developed to obtain high-resolution 3D images of large biological specimens using a synchrotron X-ray microtomography unit. It was shown that the samples (late mouse embryos) treated with 50% Lugol solution with addition of 25% ethanol for 48 h followed by a 48-h washout in phosphate buffered saline had maximum contrast and lowest compression artifacts. Processing of samples by this protocol allowed detecting zones of active proliferation. Incubation of brain samples for 120 h in 7.6% meglumine/sodium diatrizoate without washout ensured the best contrast during myelin identification.

MeSH terms

  • Animals
  • Brain / diagnostic imaging
  • Contrast Media*
  • Embryo, Mammalian / diagnostic imaging
  • Female
  • Iodides*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • X-Ray Microtomography / methods*


  • Contrast Media
  • Iodides
  • Lugol's solution