The pore-forming bacterial effector, VopQ, halts autophagic turnover

Autophagy. 2013 Dec;9(12):2169-70. doi: 10.4161/auto.26449. Epub 2013 Oct 8.

Abstract

Vibrio parahemolyticus Type III effector VopQ is both necessary and sufficient to induce autophagy within one hour of infection. We demonstrated that VopQ interacts with the Vo domain of the conserved vacuolar H(+)-ATPase. Membrane-associated VopQ subsequently forms pores in the membranes of acidic compartments, resulting in immediate release of protons without concomitant release of lumenal protein contents. These studies show how a bacterial pathogen can compromise host ion potentials using a gated pore-forming effector to equilibrate levels of small molecules found in endolysosomal compartments and disrupt cellular processes such as autophagy.

Keywords: T3SS; Vibrio; VopQ; autophagy; channel; pore; virulence factor.

MeSH terms

  • Autophagy*
  • Bacterial Proteins / metabolism*
  • Bacterial Proteins / pharmacology
  • HeLa Cells
  • Humans
  • Pore Forming Cytotoxic Proteins / metabolism*
  • Pore Forming Cytotoxic Proteins / pharmacology
  • Vibrio parahaemolyticus / metabolism
  • Vibrio parahaemolyticus / pathogenicity*
  • Virulence Factors / metabolism*
  • Virulence Factors / pharmacology

Substances

  • Bacterial Proteins
  • Pore Forming Cytotoxic Proteins
  • Virulence Factors