Ultrahigh-throughput sorting of microfluidic drops with flow cytometry

Lab Chip. 2013 Dec 7;13(23):4563-72. doi: 10.1039/c3lc50736j.

Abstract

The detection and sorting of aqueous drops is central to microfluidic workflows for high-throughput biology applications, including directed evolution, digital PCR, and antibody screening. However, high-throughput detection and sorting of drops require optical systems and microfluidic components that are complex, difficult to build, and often yield inadequate sensitivity and throughput. Here, we demonstrate a general method to harness flow cytometry, with its unmatched speed and sensitivity, for droplet-based microfluidic sorting.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle
  • DNA Primers / metabolism
  • Emulsions / chemistry
  • Flow Cytometry / instrumentation
  • Flow Cytometry / methods*
  • Fluoresceins / chemistry
  • Microfluidic Analytical Techniques / instrumentation*
  • Oils / chemistry
  • Plasmids / metabolism
  • Polymerase Chain Reaction
  • Serum Albumin, Bovine / chemistry
  • Serum Albumin, Bovine / metabolism
  • Taq Polymerase / metabolism
  • Water / chemistry

Substances

  • DNA Primers
  • Emulsions
  • Fluoresceins
  • Oils
  • Water
  • Serum Albumin, Bovine
  • 6-carboxyfluorescein
  • Taq Polymerase