Thyroid hormone regulates transcription of the gene for cytosolic phosphoenolpyruvate carboxykinase (GTP) in rat liver

Biochemistry. 1985 Aug 13;24(17):4509-12. doi: 10.1021/bi00338a004.


Using an in vitro assay with isolated rat nuclei, we have determined that thyroid hormone causes a 4-6-fold increase in the synthesis of mRNA coding for phosphoenolpyruvate carboxykinase. Proportional changes were seen in the steady-state cytosolic mRNA levels for phosphoenolpyruvate carboxykinase. Dibutyryladenosine cyclic 3',5'-monophosphate, which stimulates transcription of the phosphoenolpyruvate carboxykinase gene in normal rats, remained effective in hypo- or hyperthyroid animals. The effect of epinephrine on transcription of the gene for phosphoenolpyruvate carboxykinase appears to be modulated by thyroid hormone.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bucladesine / pharmacology
  • Cytosol / enzymology
  • Epinephrine / pharmacology
  • Genes / drug effects*
  • Liver / drug effects
  • Liver / enzymology*
  • Male
  • Phosphoenolpyruvate Carboxykinase (GTP) / genetics*
  • Poly A / genetics
  • RNA / genetics
  • RNA, Messenger / genetics
  • Rats
  • Rats, Inbred Strains
  • Thyroidectomy
  • Transcription, Genetic / drug effects*
  • Triiodothyronine / pharmacology*


  • RNA, Messenger
  • Triiodothyronine
  • Poly A
  • RNA
  • Bucladesine
  • Phosphoenolpyruvate Carboxykinase (GTP)
  • Epinephrine