Antioxidant/prooxidant effects of α-tocopherol, quercetin and isorhamnetin on linoleic acid peroxidation induced by Cu(II) and H2O2

Int J Food Sci Nutr. 2014 Mar;65(2):226-34. doi: 10.3109/09637486.2013.845654. Epub 2013 Oct 24.

Abstract

The peroxidation of linoleic acid (LA) in the presence of copper(II) (Cu(II)) ions alone and with α-tocopherol (α-TocH) was investigated in aerated and incubated emulsions at 37 °C and pH 7. Additionally, the effects of quercetin (QR) and its O-methylated derivative, isorhamnetin (IR), as potential antioxidant protectors were studied in the (Cu(II) + TocH)-induced LA peroxidation system. Cu(II)-induced LA peroxidation followed pseudo-first-order kinetics with respect to primary (hydroperoxides) and secondary (aldehydes- and ketones-like) oxidation products, which were determined by ferric thiocyanate and thiobarbituric acid-reactive substances methods, respectively. As opposed to the concentration-dependent (at 0.6 and 10.0 µM) prooxidative action of α-TocH in the absence of QR and IR, the latter two compounds showed antioxidant effect over TocH. The peroxidation of LA in the presence of Cu(II)-H(2)O(2) combination alone and with TocH, QR and IR were also investigated in aerated and incubated emulsions, where the latter three compounds exhibited antioxidant effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Copper
  • Hydrogen Peroxide
  • Linoleic Acid / metabolism*
  • Lipid Peroxidation / drug effects*
  • Oxidants / pharmacology*
  • Quercetin / analogs & derivatives*
  • Quercetin / pharmacology*
  • alpha-Tocopherol / pharmacology*

Substances

  • Antioxidants
  • Oxidants
  • 3-methylquercetin
  • Copper
  • Quercetin
  • Linoleic Acid
  • Hydrogen Peroxide
  • alpha-Tocopherol