Histamine release induced from mast cells by active components of compound 48/80

Eur J Pharmacol. 1985 Sep 24;115(2-3):163-70. doi: 10.1016/0014-2999(85)90687-9.

Abstract

Compound 48/80 and 14C-labeled compound 48/80 were synthesized, and fractionated by thin-layer chromatography into 14 components (A-N) with various histamine releasing activities and different Ca2+ requirements for their actions. The histamine release induced from rat mast cells in vitro by the most active component, fraction D (molecular weight = 2280, a tridecamer composed of 13 monomer units), was greatly enhanced by extracellular Ca2+, and was partially reduced by pretreatment of the cells with dinitrophenylated ascaris antiserum, an IgE. In contrast, the histamine release induced by fraction H (molecular weight = 1580, a nonamer composed of 9 monomer units), was higher in Ca2+ -free medium than in Ca2+-containing medium, and was partially reduced by pretreatment of mast cells with neurotensin or substance P, Ca2+-independent releasers. Apparently both fractions D and H are useful reagents for investigating the role of Ca2+ in histamine release and releaser binding in mast cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Helminth / administration & dosage
  • Ascaris / immunology
  • Autoradiography
  • Calcium / pharmacology
  • Histamine Release / drug effects*
  • Immunization
  • Immunoglobulin E / immunology
  • In Vitro Techniques
  • Isomerism
  • Male
  • Mast Cells / drug effects*
  • Molecular Weight
  • Neurotensin / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Substance P / pharmacology
  • p-Methoxy-N-methylphenethylamine / pharmacology*

Substances

  • Antigens, Helminth
  • Substance P
  • Immunoglobulin E
  • Neurotensin
  • p-Methoxy-N-methylphenethylamine
  • Calcium