Plasticity of functional epithelial polarity

Nature. 1985 Nov 28-Dec 4;318(6044):368-71. doi: 10.1038/318368a0.

Abstract

The fundamental characteristics that allow vectorial transport across an epithelial cell are the differential sorting and insertion of transport proteins either in the apical or the basolateral plasma membrane, and the preferential association of endocytosis and exocytosis with one or the other pole of the cell. Asymmetrical cellular structure and function, being manifestations of terminal differentiation, might be expected to be predetermined and invariant. Here we show that the polarity of transepithelial H+ transport, endocytosis and exocytosis in kidney can be reversed by environmental stimuli. The HCO3- secreting cell in the cortical collecting tubule is found to be an intercalated cell possessing a Cl-/HCO3- exchanger in the apical membrane and proton pumps in endocytic vesicles that fuse with the basolateral membrane; the H+-secreting cell in the medullary collecting tubule has these transport functions on the opposite membranes. Further, the HCO3- -secreting cell can be induced to change its functional polarity to that of the H+-secreting cell by acid-loading the animal.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bicarbonates / metabolism*
  • Biological Transport
  • Carbon Dioxide / blood
  • Cell Membrane / metabolism
  • Cell Membrane / ultrastructure
  • Endocytosis
  • Epithelium / metabolism
  • Epithelium / ultrastructure
  • Exocytosis
  • Fluorescent Dyes
  • Hydrogen-Ion Concentration*
  • Ion Channels / metabolism
  • Kidney Tubules / metabolism*
  • Kidney Tubules, Collecting / metabolism*
  • Kidney Tubules, Collecting / ultrastructure
  • Microscopy, Electron
  • Proton-Translocating ATPases / metabolism*

Substances

  • Bicarbonates
  • Fluorescent Dyes
  • Ion Channels
  • Carbon Dioxide
  • Proton-Translocating ATPases