Influence of corrosion on lipopolysaccharide affinity for two different titanium materials

J Prosthet Dent. 2013 Dec;110(6):462-70. doi: 10.1016/j.prosdent.2013.04.004. Epub 2013 Oct 22.


Statement of problem: Titanium is subject to corrosion in the oral cavity, which could contribute to periimplantitis. However, the effect of corrosion on the lipopolysaccharide affinity for titanium remains unknown.

Purpose: This study evaluated the role of corrosion (in artificial saliva at pHs 3, 6.5, and 9) on the lipopolysaccharide (LPS) affinity for commercially pure titanium (cp-Ti) and Ti-6Al-4V alloy.

Material and methods: Seventy-two titanium disks were anodically polarized in a controlled environment (n=9). Control specimens were not corroded. Deionized water with different concentrations of LPS (1.5, 15, and 150 μg/mL) were used to treat the disks for 24 hours to investigate LPS adherence (n=3). Then specimens were immersed in LPS-free water to evaluate LPS elution at 24, 48, and 72 hours. Data were analyzed by the 2-way, 3-way, and 3-way repeated measures ANOVA, t tests, and the Tukey honestly significant difference (HSD) tests (α=.05).

Results: A greater corrosion rate of cp-Ti and Ti-6Al-4V alloy and a higher LPS adherence to titanium surfaces (P<.05) were noted at acidic pH. The LPS affinity was higher for the Ti-6Al-4V alloy than for cp-Ti (P<.05). More LPS was eluted from titanium surfaces after a 24-hour interval.

Conclusion: Lipopolysaccharide affinity for cp-Ti and Ti-6Al-4V alloy is influenced by the corrosion process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesiveness
  • Alloys
  • Corrosion
  • Dental Alloys / chemistry*
  • Dental Materials / chemistry*
  • Diffusion
  • Electrochemical Techniques
  • Escherichia coli*
  • Humans
  • Hydrogen-Ion Concentration
  • Lipopolysaccharides / chemistry*
  • Materials Testing
  • Saliva, Artificial / chemistry
  • Surface Properties
  • Time Factors
  • Titanium / chemistry*
  • Water / chemistry


  • Alloys
  • Dental Alloys
  • Dental Materials
  • Lipopolysaccharides
  • Saliva, Artificial
  • lipopolysaccharide, E coli O55-B5
  • Water
  • titanium alloy (TiAl6V4)
  • Titanium