Activator protein-1 (AP-1) and response to pathogen infection in the Hong Kong oyster (Crassostrea hongkongensis)

Fish Shellfish Immunol. 2014 Jan;36(1):83-9. doi: 10.1016/j.fsi.2013.10.005. Epub 2013 Oct 23.

Abstract

Growing evidence suggests that the transcription factor activator protein-1 (AP-1), a downstream target of mitogen-activated protein kinase (MAPK) signaling, plays a major role in stimulating the synthesis of immune effector molecules during innate immune responses. We have characterized ChAP-1, an AP-1-like protein in Crassostrea hongkongensis that is a member of the AP-1 family of proteins. ChAP-1 is composed of 290 amino acid residues with a Jun and bZIP domain at the N- and C-termini, respectively, a structure similar to that of known Ap-1 proteins. ChAP-1 mRNA is expressed in several tissues analyzed, with highest expression in the mantle. Expression of ChAP-1 increases in response to Vibrio alginolyticus, Salmo haemolyticus or Salmo cerevisiae infection and, despite the location of GFP-tagged full-length ChAP-1 protein in the cytoplasm, ChAP-1 activates the transcription of an L8G5-luc reporter gene, and its over-expression can also activate the AP-1-Luc reporter gene in HEK293T cells.

Keywords: ChAP-1; Crassostrea hongkongensis; Immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Crassostrea / genetics
  • Crassostrea / immunology*
  • Crassostrea / virology
  • HeLa Cells
  • Humans
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Phylogeny*
  • RNA / chemistry
  • RNA / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Transcription Factor AP-1 / genetics
  • Transcription Factor AP-1 / immunology*
  • Transfection / methods
  • Vibrio Infections / immunology*
  • Vibrio Infections / virology
  • Vibrio alginolyticus / immunology*

Substances

  • Transcription Factor AP-1
  • RNA

Associated data

  • GENBANK/KC890768